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Tissue Culture Technician
Tissue culture technicians use various techniques to
asexually reproduce live tissue (under laboratory conditions)
to produce specimens which are genetically identical to, or
different from, the parent tissue as required.
This method of reproduction is commonly known as cloning.
Tissue culture is used for a wide variety of commercially
important agricultural and horticultural plants, in the
propagation of rare and endangered plant species, and in
health and medical research and treatments.
A tissue culture technician may perform the following
tasks:
- set up equipment
- clean, sterilise and maintain equipment and facilities
- select live material with the desired characteristics
- dissect, sterilise, test for viruses and other diseases,
and otherwise prepare selected tissues for reproduction
- prepare reproductive and growth chemical solutions
suitable for the species to be cloned
- chemically treat selected tissues to induce genetic
mutations
- place tissues in sterile chemical solutions and place on
shakers to initiate reproduction or growth
- place reproduced material in sealed sterile containers
containing a nutrient gel, grow specimens under controlled
temperature and light conditions - repeating the process
until they are of sufficient size
- record results by using computers or other methods
- grow samples of cloned organisms to maturity and monitor
them for desirable or undesirable genetic mutation.
Tissue culture technicians mostly work in laboratories,
with a small amount of field and shadehouse work. Maintenance
of sterile conditions in the laboratory is extremely
important.
Required Skills - Tissue Culture Technician
- have good eyesight and be good with hands for dissection
work and use of other laboratory equipment
- able to concentrate on detail
- able to keep records accurately
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